Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.
Identifieur interne : 000027 ( Main/Exploration ); précédent : 000026; suivant : 000028Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.
Auteurs : Huimin Cao [République populaire de Chine] ; Cuiping Wang [République populaire de Chine] ; Haibin Liu [République populaire de Chine] ; Weili Jia [République populaire de Chine] ; Hongwen Sun [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2020.
Abstract
The enzyme activities of the fungus Lasiodiplodia theobromae (L. theobromae) were studied during degradation of benzo[a]pyrene (BaP). The L. theobromae was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil collected from the Beijing Coking Plant in China and can potentially use BaP as its sole carbon source with a degradation ratio of up to 53% over 10 days. The activities of lignin peroxidase (LiP) and laccase (LAC) could be detected during BaP biodegradation; while manganese peroxidase (MnP) was not detected. Both glucose and salicylic acid enhanced BaP biodegradation slightly. In contrast, the coexistence of phenanthrene (PHE) inhibited BaP degradation. These metabolic substrates all enhanced the secretion of LiP and LAC. The addition of Tween 80 (TW-80) enhanced BaP biodegradation as well as the LiP and LAC activities. At the same time, TW-80 was degraded by the L. theobromae. In addition, the L. theobromae was compared to Phanerochaete chrysosporium (P. chrysosporium), which is a widely studied fungus for degrading PAH. P. chrysosporium was unable to use BaP as its sole carbon source. The activities of LiP and LAC produced by the P. chrysosporium were less than those of the L. theobromae. Additionally, the four intermediates formed in the BaP biodegradation process were monitored using GC-MS analysis. Four metabolite concentrations first increased and then decreased or obtained the platform with prolonged BaP biodegradation time. Therefore, this study shows that the L. theobromae may be explored as a new strain for removing PAHs from the environment.
DOI: 10.1038/s41598-020-57692-6
PubMed: 31964981
PubMed Central: PMC6972742
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Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071</wicri:regionArea><wicri:noRegion>300071</wicri:noRegion></affiliation></author><author><name sortKey="Jia, Weili" sort="Jia, Weili" uniqKey="Jia W" first="Weili" last="Jia">Weili Jia</name><affiliation wicri:level="1"><nlm:affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071</wicri:regionArea><wicri:noRegion>300071</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Hongwen" sort="Sun, Hongwen" uniqKey="Sun H" first="Hongwen" last="Sun">Hongwen Sun</name><affiliation wicri:level="1"><nlm:affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China. sunhongwen@nankai.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071</wicri:regionArea><wicri:noRegion>300071</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno 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The L. theobromae was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil collected from the Beijing Coking Plant in China and can potentially use BaP as its sole carbon source with a degradation ratio of up to 53% over 10 days. The activities of lignin peroxidase (LiP) and laccase (LAC) could be detected during BaP biodegradation; while manganese peroxidase (MnP) was not detected. Both glucose and salicylic acid enhanced BaP biodegradation slightly. In contrast, the coexistence of phenanthrene (PHE) inhibited BaP degradation. These metabolic substrates all enhanced the secretion of LiP and LAC. The addition of Tween 80 (TW-80) enhanced BaP biodegradation as well as the LiP and LAC activities. At the same time, TW-80 was degraded by the L. theobromae. In addition, the L. theobromae was compared to Phanerochaete chrysosporium (P. chrysosporium), which is a widely studied fungus for degrading PAH. P. chrysosporium was unable to use BaP as its sole carbon source. The activities of LiP and LAC produced by the P. chrysosporium were less than those of the L. theobromae. Additionally, the four intermediates formed in the BaP biodegradation process were monitored using GC-MS analysis. Four metabolite concentrations first increased and then decreased or obtained the platform with prolonged BaP biodegradation time. Therefore, this study shows that the L. theobromae may be explored as a new strain for removing PAHs from the environment.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31964981</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>21</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.</ArticleTitle><Pagination><MedlinePgn>865</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-020-57692-6</ELocationID><Abstract><AbstractText>The enzyme activities of the fungus Lasiodiplodia theobromae (L. theobromae) were studied during degradation of benzo[a]pyrene (BaP). The L. theobromae was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil collected from the Beijing Coking Plant in China and can potentially use BaP as its sole carbon source with a degradation ratio of up to 53% over 10 days. The activities of lignin peroxidase (LiP) and laccase (LAC) could be detected during BaP biodegradation; while manganese peroxidase (MnP) was not detected. Both glucose and salicylic acid enhanced BaP biodegradation slightly. In contrast, the coexistence of phenanthrene (PHE) inhibited BaP degradation. These metabolic substrates all enhanced the secretion of LiP and LAC. The addition of Tween 80 (TW-80) enhanced BaP biodegradation as well as the LiP and LAC activities. At the same time, TW-80 was degraded by the L. theobromae. In addition, the L. theobromae was compared to Phanerochaete chrysosporium (P. chrysosporium), which is a widely studied fungus for degrading PAH. P. chrysosporium was unable to use BaP as its sole carbon source. The activities of LiP and LAC produced by the P. chrysosporium were less than those of the L. theobromae. Additionally, the four intermediates formed in the BaP biodegradation process were monitored using GC-MS analysis. Four metabolite concentrations first increased and then decreased or obtained the platform with prolonged BaP biodegradation time. Therefore, this study shows that the L. theobromae may be explored as a new strain for removing PAHs from the environment.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Huimin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Cuiping</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China. wangcp@nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Haibin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Weili</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Hongwen</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China. sunhongwen@nankai.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">24657347</ArticleId></ArticleIdList></Reference><Reference><Citation>J Hazard Mater. 2009 Sep 30;169(1-3):1-15</Citation><ArticleIdList><ArticleId IdType="pubmed">19442441</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1986 Dec 25;261(36):16948-52</Citation><ArticleIdList><ArticleId IdType="pubmed">3023375</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16593451</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 1996 Oct;46(3):313-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8933845</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Pollut. 2008 Feb;151(3):669-77</Citation><ArticleIdList><ArticleId IdType="pubmed">17482734</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2009 Jun;100(12):3157-60</Citation><ArticleIdList><ArticleId IdType="pubmed">19246189</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2002 Oct;60(1-2):212-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12382066</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 1998 Jan 20;57(2):220-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10099197</ArticleId></ArticleIdList></Reference><Reference><Citation>J Hazard Mater. 2009 Jun 15;165(1-3):1229-33</Citation><ArticleIdList><ArticleId IdType="pubmed">19010597</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1985 Jun 21;228(4706):1434-6</Citation><ArticleIdList><ArticleId IdType="pubmed">3925550</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Res Int. 2012;2012:243217</Citation><ArticleIdList><ArticleId IdType="pubmed">22830035</ArticleId></ArticleIdList></Reference><Reference><Citation>J Hazard Mater. 2003 Dec 12;105(1-3):157-67</Citation><ArticleIdList><ArticleId IdType="pubmed">14623425</ArticleId></ArticleIdList></Reference><Reference><Citation>J Environ Manage. 2012 Nov 30;111:115-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22835655</ArticleId></ArticleIdList></Reference><Reference><Citation>3 Biotech. 2014 Apr;4(2):205-211</Citation><ArticleIdList><ArticleId IdType="pubmed">28324451</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2003 Jul;69(7):3957-64</Citation><ArticleIdList><ArticleId IdType="pubmed">12839767</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Sci Pollut Res Int. 2014 Sep;21(18):10614-25</Citation><ArticleIdList><ArticleId IdType="pubmed">24878554</ArticleId></ArticleIdList></Reference><Reference><Citation>Langmuir. 2007 Apr 10;23(8):4130-6</Citation><ArticleIdList><ArticleId IdType="pubmed">17348695</ArticleId></ArticleIdList></Reference><Reference><Citation>J Environ Sci (China). 2009;21(10):1446-51</Citation><ArticleIdList><ArticleId IdType="pubmed">20000001</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Pollut. 2012 May;164:169-74</Citation><ArticleIdList><ArticleId IdType="pubmed">22361056</ArticleId></ArticleIdList></Reference><Reference><Citation>Chemosphere. 2007 Apr;67(7):1368-74</Citation><ArticleIdList><ArticleId IdType="pubmed">17126885</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2016 Sep;216:1102-5</Citation><ArticleIdList><ArticleId IdType="pubmed">27342606</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2001 Oct;57(1-2):20-33</Citation><ArticleIdList><ArticleId IdType="pubmed">11693920</ArticleId></ArticleIdList></Reference><Reference><Citation>Chemosphere. 2009 Oct;77(6):733-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19751947</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1986 May 25;261(15):6900-3</Citation><ArticleIdList><ArticleId IdType="pubmed">3700421</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1994 Feb;60(2):459-66</Citation><ArticleIdList><ArticleId IdType="pubmed">16349176</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Pollut. 1993;81(3):229-49</Citation><ArticleIdList><ArticleId IdType="pubmed">15091809</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1996 Dec;62(12):4563-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16535468</ArticleId></ArticleIdList></Reference><Reference><Citation>Water Res. 2019 Sep 1;160:238-248</Citation><ArticleIdList><ArticleId IdType="pubmed">31152949</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 1995 Mar;42(6):958-63</Citation><ArticleIdList><ArticleId IdType="pubmed">7766094</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Cao, Huimin" sort="Cao, Huimin" uniqKey="Cao H" first="Huimin" last="Cao">Huimin Cao</name></noRegion><name sortKey="Jia, Weili" sort="Jia, Weili" uniqKey="Jia W" first="Weili" last="Jia">Weili Jia</name><name sortKey="Liu, Haibin" sort="Liu, Haibin" uniqKey="Liu H" first="Haibin" last="Liu">Haibin Liu</name><name sortKey="Sun, Hongwen" sort="Sun, Hongwen" uniqKey="Sun H" first="Hongwen" last="Sun">Hongwen Sun</name><name sortKey="Wang, Cuiping" sort="Wang, Cuiping" uniqKey="Wang C" first="Cuiping" last="Wang">Cuiping Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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